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Article Title: Distribution of Bifidobacterial Species in Human Intestinal Microflora Examined with 16S rRNA-Gene-Targeted Species-Specific Primers

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Figure Lengend Snippet: Bacterial strains and results of PCR assays in which species-specific primers BiLON, BiINF, BiDEN, and BiGAL were used a

Article Snippet: Six edge fields and four center fields were counted, and the counts were then correlated with the actual sample size ( 9 ). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Species Strain(s) b Species-specific primer PCR results c BiLON BiINF BiDEN BiGAL B. adolescentis ATCC 15703 T , NCFB 2229, NCFB 2230, NCFB 2231 − − − − B. angulatum ATCC 27535 T , JCM 1252 − − − − B. bifidum ATCC 29521 T , ATCC 15696, ATCC 11863, Yakult d − − − − B. breve ATCC 15700 T , ATCC 15698, ATCC 15701, Yakult d − − − − B. catenulatum ATCC 27539 T , JCM 7130 − − − − B. pseudocatenulatum JCM 1200 T , DSM 20439 − − − − B. longum ATCC 15707 T , ATCC 15708, FERM P-6548 + − − − B. infantis ATCC 15697 T , ATCC 15702, ATCC 25962 − + − − B. suis ATCC 27533 T + − − − B. dentium ATCC 27534 T , DSM 20084, DSM 20221 − − + − B. gallicum JCM 8224 T − − − + Open in a separate window a In addition to the bacteria listed, negative PCR results with primers BiLON, BiINF, BiDEN, and BiGAL were obtained for the following bacterial species: B. animalis ATCC 25527 T , B. asteroides ATCC 25910 T , B. boum JCM 1211 T , B. choerinum ATCC 27686 T , B. coryneforme ATCC 25911 T , B. cuniculi ATCC 27916 T , B. denticolens DSM 10105 T , B. gallinarum JCM 6291 T , B. indicum ATCC 25912 T , B. inopinatum DSM 10107 T , B. lactis DSM 10140 T , B. magnum JCM 1218 T , B. merycicum JCM 8219 T , B. minimum ATCC 27538 T , B. pseudolongum subsp. globosum ATCC 25864 T , B. pseudolongum subsp. pseudolongum JCM 1205 T , B. pullorum JCM 1214 T , B. ruminantium JCM 8222 T , B. saeculare DSM 6531 T , B. subtile DSM 20096 T , B. thermophilium ATCC 25866 T , E. coli ATCC 11775 T , Bacteroides fragilis NCTC 9343 T , Bacteroides ovatus JCM 5824 T , Bacteroides vulgatus ATCC 8424 T , Clostridium bifermentans JCM 1386 T , Clostridium perfringens JCM 1290 T , Enterococcus faecalis ATCC 19433 T , Enterococcus faecium ATCC 19434 T , Eubacterium aerofaciens ATCC 25986 T , Eubacterium biforme ATCC 27806 T , Gardnerella vaginalis DSM 4944 T , Lactobacillus acidophilus ATCC 4356 T , Propionibacterium acnes ATCC 6919 T , Peptostreptococcus prevotii ATCC 9321 T , and Ruminococcus productus ATCC 27340 T . b ATCC, American Type Culture Collection; JCM, Japan Collection of Microorganisms; DSM, German Collection of Microorganisms and Cell Cultures; NCFB, National Collection of Food Bacteria; NCTC, National Collection of Type Cultures; FERM, National Institute of Biosciences and Human Technology. c The PCR specificities of other species-specific primers, such as BiADO, BiANG, BiBIF, BiBRE, and BiCATg, have been reported previously ( 16 ). d These strains are used in a probiotic culture in dairy products and were obtained from Yakult Central Institute for Microbiological Research.

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